Losmanova T, Tschan MP, Galván JA, Berezowska S
Published in Methods Mol Biol. 2023, 2566:141-147. doi: 10.1007/978-1-0716-2675-7_11. PMID: 36152248
Abstract
Autophagy is crucial for maintaining cellular homeostasis and its deregulation is involved in disease development, including cancer. The key players of chaperone-mediated autophagy (CMA), a particular selective subtype of autophagy, are HSPA8 and LAMP2A. Both proteins can be immunohistochemically detected in formalin-fixed paraffin-embedded (FFPE) tissue. LAMP2A is frequently overexpressed in a variety of cancers where it likely supports cancer cell survival and resistance to anti-cancer therapies in a context-dependent manner. Here we present the immunohistochemical staining protocol of antibodies against LAMP2A and HSPA8, using an automated staining system, suitable for routine diagnostics. Additionally, we also suggest a staining evaluation method.
Keywords: CMA; Chaperone-mediated autophagy; HSPA8; Immunohistochemistry; LAMP2A.
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